GK041 ScienCell 人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞，細胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量，實現(xiàn)細胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進口，貨期穩(wěn)定，折扣好的服務標準。

GK041 ScienCell人體皮膚傷口愈合qPCR檢測試劑盒，Human Skin Wound Healing qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號：GK041

產(chǎn)品名稱：GK041 ScienCell人體皮膚傷口愈合qPCR檢測試劑盒，Human Skin Wound Healing qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK041 ScienCell人體皮膚傷口愈合qPCR檢測試劑盒，Human Skin Wound Healing qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人類皮膚傷口愈合 qPCR 陣列試劑盒 （GQH-SWH） 旨在促進傷口修復過程中涉及的 88 個關(guān)鍵基因的基因表達譜分析。傷口愈合是一個復雜的過程，包括許多生物事件，如吞噬作用、血管生成、新膠原生成、上皮化和膠原蛋白重塑。一般可分為3個階段：1）止血凝血，2）炎癥，3）增殖和組織重塑。如何根據(jù)其功能對所包含的基因進行分組的簡要示例如下所示：  

傷口愈合階段

止血和凝血： IL1A， IL1B， IL6， TNF， FGF2， IGF1， TGFs， HIF1A

炎癥：CXCL8，VEGFs，ELANE，CTSG，PRTN3，PLAU，TGF，PDGF，TNF，IFNG

增殖和組織重塑：EGF，FGF7，IGF1，IFNG，CSK，RAC1，RHOA，CDC42

細胞外基質(zhì)成分：COL1A2、COL3A1、COL4A1、COL14A1

細胞粘附：CDH1，ITGA，ITGB，MMP，F3，FGA，TIMP1，ACTA2，ACTC1，TAGLN

細胞因子和趨化因子：CCL2、CXCL1、CXCL2、CXCL8、IL1A、IL1B、IL4、IL6

生長因子：腦脊液2，腦脊液3，EGF，CTGF，FGF，PDGF，TGF

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Wound healing stages

o    Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A

o    Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG

o    Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42

·         Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1

·         Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN

·         Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6

·         Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人體皮膚傷口愈合qPCR陣列板布局*（8 個粗體和斜體控件）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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